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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by <t>ELISA.</t> Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.
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Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by ELISA. Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.

Journal: International Journal of Molecular Sciences

Article Title: Modulation of the miR-485-3p/PGC-1α Pathway by ASO-Loaded Nanoparticles Attenuates ALS Pathogenesis

doi: 10.3390/ijms27020615

Figure Lengend Snippet: Evaluation of NfL concentration changes in CSF of SOD1 G93A transgenic mouse model. ( A ) Schematic procedure to measure NfL concentration in CSF in SOD1 G93A mouse model. ( B ) NfL variation was validated by ELISA. Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. vehicle-treated WT or SOD1 G93A group; n = 4–5 per group.

Article Snippet: PGC-1α protein levels were measured using a mouse-specific ELISA kit (Cat #E1487Mo, BTLab, Shanghai, China) in accordance with the manufacturer’s instruction.

Techniques: Concentration Assay, Transgenic Assay, Enzyme-linked Immunosorbent Assay